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The minimum inhibitory concentration (MIC, μg/mL) of ebselen and control antibiotics against enterococci clinical isolates.
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The minimum inhibitory concentration (MIC, μg/mL) of ebselen and control antibiotics against enterococci clinical isolates.
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The minimum inhibitory concentration (MIC, μg/mL) of ebselen and control antibiotics against enterococci clinical isolates.

Journal: PLoS ONE

Article Title: Repurposing ebselen for decolonization of vancomycin-resistant enterococci (VRE)

doi: 10.1371/journal.pone.0199710

Figure Lengend Snippet: The minimum inhibitory concentration (MIC, μg/mL) of ebselen and control antibiotics against enterococci clinical isolates.

Article Snippet: The minimum inhibitory concentration (MIC) of ebselen and control antibiotics (linezolid, ramoplanin and vancomycin) was assessed in accordance with the guidelines outlined by the Clinical and Laboratory Standards Institute (CLSI) [ ].

Techniques: Concentration Assay, Isolation

(A) Biofilm inhibition activity of ebselen . Sub-inhibitory concentrations of each drug were added to bacteria in tryptic soya broth (TSB) + 1% glucose and incubated for 24 hours at 37 °C. The biofilm mass (OD 595 ) was measured after staining with crystal violet and destaining with ethanol. Data is presented as biofilm mass relative to DMSO-treated wells, (*) indicates significant difference from DMSO treated wells using 2-way ANOVA with Dunnett’s pairwise comparison (P< 0.001). (B) Biofilm eradication activity of ebselen . Bacteria were incubated for 24 hours in TSB + 1% glucose to allow for the formation of mature biofilm. Supra-inhibitory concentrations of the drugs were then added and incubated with the bacterial biofilm for additional 24 hours before the biofilm density was measured (OD 595 ) by crystal violet staining. Data is presented as biofilm mass relative to DMSO-treated wells, (*) indicates significant difference from linezolid-treated wells, while ( # ) indicates significant difference from ramoplanin-treated wells using 2-way ANOVA with Dunnett’s post hoc comparison test at P<0.05.

Journal: PLoS ONE

Article Title: Repurposing ebselen for decolonization of vancomycin-resistant enterococci (VRE)

doi: 10.1371/journal.pone.0199710

Figure Lengend Snippet: (A) Biofilm inhibition activity of ebselen . Sub-inhibitory concentrations of each drug were added to bacteria in tryptic soya broth (TSB) + 1% glucose and incubated for 24 hours at 37 °C. The biofilm mass (OD 595 ) was measured after staining with crystal violet and destaining with ethanol. Data is presented as biofilm mass relative to DMSO-treated wells, (*) indicates significant difference from DMSO treated wells using 2-way ANOVA with Dunnett’s pairwise comparison (P< 0.001). (B) Biofilm eradication activity of ebselen . Bacteria were incubated for 24 hours in TSB + 1% glucose to allow for the formation of mature biofilm. Supra-inhibitory concentrations of the drugs were then added and incubated with the bacterial biofilm for additional 24 hours before the biofilm density was measured (OD 595 ) by crystal violet staining. Data is presented as biofilm mass relative to DMSO-treated wells, (*) indicates significant difference from linezolid-treated wells, while ( # ) indicates significant difference from ramoplanin-treated wells using 2-way ANOVA with Dunnett’s post hoc comparison test at P<0.05.

Article Snippet: The minimum inhibitory concentration (MIC) of ebselen and control antibiotics (linezolid, ramoplanin and vancomycin) was assessed in accordance with the guidelines outlined by the Clinical and Laboratory Standards Institute (CLSI) [ ].

Techniques: Inhibition, Activity Assay, Incubation, Staining

Infected mice were orally treated with ebselen (0.5 mg/kg) and ramoplanin (10 mg/kg) daily for 8 days. One group was left untreated. Fecal samples were freshly collected from mice in each group on days 0, 5, 10, 15, and 20 post treatment. (*) denotes significant difference from the untreated group (P < 0.001).

Journal: PLoS ONE

Article Title: Repurposing ebselen for decolonization of vancomycin-resistant enterococci (VRE)

doi: 10.1371/journal.pone.0199710

Figure Lengend Snippet: Infected mice were orally treated with ebselen (0.5 mg/kg) and ramoplanin (10 mg/kg) daily for 8 days. One group was left untreated. Fecal samples were freshly collected from mice in each group on days 0, 5, 10, 15, and 20 post treatment. (*) denotes significant difference from the untreated group (P < 0.001).

Article Snippet: The minimum inhibitory concentration (MIC) of ebselen and control antibiotics (linezolid, ramoplanin and vancomycin) was assessed in accordance with the guidelines outlined by the Clinical and Laboratory Standards Institute (CLSI) [ ].

Techniques: Infection

Infected mice (n = 5 per group) were orally treated with ebselen (0.5 mg/kg) or ramoplanin (10 mg/kg) daily for eight days. One group was left untreated. Cecum and ileum contents were collected one day after the last treatment was administered (day 21 of experiment). Asterisk (*) denotes significant difference from the untreated group (P < 0.05). No significant difference was found between ebselen-treated and ramoplanin-treated groups.

Journal: PLoS ONE

Article Title: Repurposing ebselen for decolonization of vancomycin-resistant enterococci (VRE)

doi: 10.1371/journal.pone.0199710

Figure Lengend Snippet: Infected mice (n = 5 per group) were orally treated with ebselen (0.5 mg/kg) or ramoplanin (10 mg/kg) daily for eight days. One group was left untreated. Cecum and ileum contents were collected one day after the last treatment was administered (day 21 of experiment). Asterisk (*) denotes significant difference from the untreated group (P < 0.05). No significant difference was found between ebselen-treated and ramoplanin-treated groups.

Article Snippet: The minimum inhibitory concentration (MIC) of ebselen and control antibiotics (linezolid, ramoplanin and vancomycin) was assessed in accordance with the guidelines outlined by the Clinical and Laboratory Standards Institute (CLSI) [ ].

Techniques: Infection